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Office
2845 Niel
Belgium
Starting off on the right foot.
At ImmuneSpec, we begin by understanding your project goals in a discussion. Our discovery meeting covers project type, target peptides (known/unknown), HLA-type of interest and required input material. Following this, we prepare for the project, set-up experimental design and establish the databases.
During the kickoff, we usually discuss:
Project information
Sample details
- Deadlines
- Objectives
- Specific requirements
- Sample intake details
- Sample amounts
- Other additional data/ materials
Prepping well, is half the work.
After the project kickoff, the second step involves delivering your samples to us. As the nature of the samples varies depending on the specific project, we always make sure to discuss storage, handling and (bio)safety requirements.
Project information
- How will the samples be prepared
- Requirements for immunogenicity testing, neo-antigen discovery or vaccine development
Analyzing the samples.
After purification, immune peptides are measured by using a TimsTOF SCP Instrument coupled to an Evosep One chromatography system. Following the measurements, advanced algorithms are deployed to match the obtained MS spectra with the corresponding protein sequence in the database.
Lab hardware
Lab software
- Evosep One
- TimsTOF SCP
- Peaks 11
- Spider search
- Western blot analysis
- Size distribution of identified peptides
- Amount of immunepeptides that match with reference proteins
- MHC Motif clustering
Project information
Answers to your questions.
Upon delivery of the report, a dedicated meeting is scheduled to present the results and guide you through the report, elucidate the significance of the obtained findings, provide expert interpretation, and offer valuable recommendations for the next steps in advancing your project.
Results & files overview
Experimental set-up overview
- List of peptides identified
- List of identified peptides matching target proteins
- Access to complete database
- Sample overview per experiment
- Western blot analysis
- Peptide size distribution
- MHC Motif clustering
Project information
Interpretation & presentation.
Upon delivery of the report, a dedicated meeting is scheduled to present the results and guide you through the report, elucidate the significance of the obtained findings, provide expert interpretation, and offer valuable recommendations for the next steps in advancing your project.
