Precision medicine &  immunotherapy

Precise identification of disease-specific (neo)antigens.

Get a full overview of detected antigens and select those with the highest potential as immunotherapeutic or for the development of precision medicine.

Select high-quality candidates

Detect disease-specific antigens with high precision & accuracy

Speed up the R&D process

Get results fast & reliable.

Expert support on demand

Get recommendations from experts with over 10+ years of experience

About precision medicine & immunotherapeutics

Precision medicine tailors medical treatments to individual patients by analyzing their unique genes and proteins, often in strong relation to their immune system. Therapeutics are designed based on these unique features, resulting in potentially more effective treatments with fewer side effects.

Precision medicine is particularly useful in treating diseases with complex causes, such as cancer. By analyzing a patient's unique proteomic profile, precision medicine can target specific mutations that are characteristic of the patient, specifically attacking diseased cells while minimizing damage to healthy cells. We can use the information about which immune-related proteins are presented on the surface of diseased cells to create highly targeted immunotherapeutics.

Immunopeptidomics for the improvement of immunotherapeutics

Immunopeptidomics involves the identification and characterization of antigen fragments (immunopeptides) loaded intracellularly on major histocompatibility complex (MHC) molecules and presented extracellularly to T cells.

With a renewed approach to immunopeptidomics, ImmuneSpec is able to detect more immunopeptides with greater precision compared to conventional methods. This leads to a more comprehensive and detailed overview of disease-specific immunopeptides unlocking their immunogenic potential.

ImmuneSpec's advanced immunopeptidomics platform serves as a powerful tool to enhance the efficacy and precision of targeted (immuno)therapies.

High numbers of immunopeptides presented by both MHC I (HLA-A/B/C) and MHC II (HLA-DR/DP/DQ) complexes can be assessed while only requiring minimal sample inputs. Even the smallest alterations in mutated proteins can be identified, enabling the selection of high-quality disease targets for the development of ultraprecise personalized cancer treatments.

Collaborating with ImmuneSpec

A renewed approach to unlock immunogenic regions of immunopeptides for the development of safe, effective vaccines and therapeutics.

Day 0

Project initiation meeting

Discuss project needs & prior research.

Starting off on the right foot.

At ImmuneSpec, we begin by understanding your project goals in a meeting between you and our project team. This meeting covers project type, target peptides (known/unknown), HLA-type of interest and required input material. This ensures delivery of optimal results and addressing the research question effectively.

During the initiation meeting, we usually discuss:

Project information

Sample details

  • Deadlines
  • Objectives
  • Specific requirements
  • Sample intake details
  • Sample amounts
  • Other additional data/ materials
Day 1-14

Sample intake & preparation

Preparing samples & lab setup.

Prepping well, is half the work.

After the project kickoff, the second step involves delivering your samples to us or through one of our affiliated partners. As the nature of the samples varies depending on the specific project, we always make sure to discuss storage, handling and (bio)safety requirements.

Project information

  • How will the samples be prepared
  • Requirements for immunogenicity testing, neoantigen discovery or vaccine development
Day 15-28

Affinity  purification and MS analysis

Executing on the laboratory work and data analysis.

Analyzing the samples.

After  purification, immune peptides are measured by using a TimsTOF SCP Instrument  coupled to an Evosep One chromatography system. Following the measurements, advanced algorithms are deployed to match the obtained MS  spectra with the corresponding protein sequence in the database.

Lab hardware

Lab software

  • Evosep One
  • TimsTOF SCP
  • Peaks 11
  • Spider search
  • Western blot analysis
  • Size distribution of identified peptides
  • Amount of immunepeptides that match with reference proteins
  • MHC Motif clustering

Project information

Day 28-35

Report generation

Turning data into ready-to-use insights.

Answers to your questions.

Upon delivery of the report, a dedicated meeting is scheduled to present the results and guide you through the report, elucidate the significance of the obtained findings, provide expert interpretation, and offer valuable recommendations for the next steps in advancing your project.

Results & files overview

Experimental set-up overview

  • List of peptides identified
  • List of identified peptides matching target proteins
  • Access to complete database
  • Sample overview per experiment
  • Western blot analysis
  • Peptide size distribution
  • MHC Motif clustering

Project information

Day 28-35

Discussion & next steps

Closing up, and onward.

Interpretation & presentation.

Upon delivery of the report, a dedicated meeting is scheduled to present the results and guide you through the report, elucidate the significance of the obtained findings, provide expert interpretation, and offer valuable recommendations for the next steps in advancing your project.
Upon request, the written report can be tailored based on the outcomes of this meeting.

"I  was blown away by the level of expertise, the great communication, thought partnership and superior results. ImmuneSpec was able to handle highly complex experimental designs, large numbers of samples and provide the highest peptide yields, while being faster than any other company we have worked with in the past."

Daniel Leventhal

Generate Biomedicines

Because antigen discovery for the large and complex parasite ‘Leishmania’ has known a deadlock for many decades, we approached the ImmuneSpec team to undertake immunopeptidomics analysis on skin biopsies and organ aspiration samples from leishmania patients. They tackled the challenging starting material with great enthusiasm and successfully created the first-ever mapping of biologically presented peptides in leishmaniasis patients. This will significantly expedite vaccine development for this neglected tropical disease affecting remote and impoverished communities.

Wim Adriaensen

Institute of Tropical Medicine Antwerp

“We are excited to partner with an outstanding team of scientists who provide cutting-edge immunopeptidomics services with remarkable flexibility and timely delivery”

Sofie Pattijn


"Encouraged by recent publications that many lncRNA-derived peptides could be more precisely detected with HLA-I peptidomics, our team reached out to the ImmuneSpec team. We selected the team among a range of companies as they provide full service - from MHC complex purification to refining MS results for identification of peptides with highest quality. They have access to one of the most precise MS instruments for such specific scientific questions and are very dedicated to give exhaustive information and are very proactive on all the steps of analysis."

Antonin Morillon

Institut Curie

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