Smoother development of safe, effective vaccines.
Find and select high quality antigens faster for an efficient, smooth vaccine development process.
Select high quality candidates
Develop effective broad-spectrum vaccines that contain multiple high potential immunogenic protein fragments of the target pathogen
Predict immune responses
Understand which immunopeptides are presented by different patient groups.
Speed up the R&D process
Run analyses fast, with guaranteed results at speed.
About the development of safe vaccines
Vaccine development aims to prevent the spread of infectious diseases by providing immunity to individuals against specific pathogens. Despite their emerging importance and the introduction of innovative technologies, the development of vaccines remains a complex and challenging process.
Overall, the identification of effective antigen fragments that ensure broad-spectrum protection against the target pathogen along with the high costs and the high risk of failure, tend to complicate the process.
Optimizing vaccinee development with immunopeptidomics
Immunopeptidomics involves the identification and characterization of peptides (antigens) presented by major histocompatibility complex (MHC) molecules.
ImmuneSpec’s advanced immunopeptidomics platform allows thorough analysis of all pathogenic immunogenic protein fragments in a faster and more accurate way.
By analyzing the ensemble of immune peptides presented by infected cells or in samples from infected patients, researchers can identify the best epitopes for the development of broad-spectrum vaccines against the target pathogen. By incorporating both MHC I and MHC II associated peptides, the resulting vaccine will trigger robust CD4 and CD8 responses.
This approach optimizes vaccine development and selects high-quality leads through the identification of true in vivo antigens. Ultimately, it leads to a more efficient research and development stage, smoother clinical trials, and faster market approval.
Starting off on the right foot.
At ImmuneSpec, we begin by understanding your project goals in a discussion. Our discovery meeting covers project type, target peptides (known/unknown), HLA-type of interest and required input material. Following this, we prepare for the project, set-up experimental design and establish the databases.
During the kickoff, we usually discuss:
Project information
Sample details
- Deadlines
- Objectives
- Specific requirements
- Sample intake details
- Sample amounts
- Other additional data/ materials
Prepping well, is half the work.
After the project kickoff, the second step involves delivering your samples to us. As the nature of the samples varies depending on the specific project, we always make sure to discuss storage, handling and (bio)safety requirements.
Project information
- How will the samples be prepared
- Requirements for immunogenicity testing, neo-antigen discovery or vaccine development
Analyzing the samples.
After purification, immune peptides are measured by using a TimsTOF SCP Instrument coupled to an Evosep One chromatography system. Following the measurements, advanced algorithms are deployed to match the obtained MS spectra with the corresponding protein sequence in the database.
Lab hardware
Lab software
- Evosep One
- TimsTOF SCP
- Peaks 11
- Spider search
- Western blot analysis
- Size distribution of identified peptides
- Amount of immunepeptides that match with reference proteins
- MHC Motif clustering
Project information
Answers to your questions.
Upon delivery of the report, a dedicated meeting is scheduled to present the results and guide you through the report, elucidate the significance of the obtained findings, provide expert interpretation, and offer valuable recommendations for the next steps in advancing your project.
Results & files overview
Experimental set-up overview
- List of peptides identified
- List of identified peptides matching target proteins
- Access to complete database
- Sample overview per experiment
- Western blot analysis
- Peptide size distribution
- MHC Motif clustering
Project information
Interpretation & presentation.
Upon delivery of the report, a dedicated meeting is scheduled to present the results and guide you through the report, elucidate the significance of the obtained findings, provide expert interpretation, and offer valuable recommendations for the next steps in advancing your project.
Daniel Leventhal
Generate Biomedicines
Wim Adriaensen
Institute of Tropical Medicine Antwerp
Sofie Pattijn
ImmunXperts
Antonin Morillon
Institut Curie
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